Autors: SPIRO M. KONSTANTINOV., Mindova, M. M., PANAYOT T. GOSPODINOV., PENKA I. GENOVA.
Title: Three-Dimensional Bioreactor Cultures: A Useful Dynamic Model for the Study of Cellular Interactions
Keywords: rotating-vessel miniPERM, bioreactor systems, culture conditions

Abstract: The ex vivo expansion of hematopoietic cells is a developing area with emphasis on bioreactor systems for amelioration of culture conditions. A rational design of bioreactors, especially those allowing microgravity, could permit the production of stem cells and will offer new approaches for studying the mechanisms of proliferation, differentiation, and signal transduction of cultured cells. The efficacy of two commercially available bioreactors (rotating-vessel miniPERM and static INTEGRA CL 350) to support long-term bone marrow cell cultures (LTBMCC) and three-dimensional growth of Hodgkin's lymphoma HD-MY-Z cells was investigated. In the miniPERM system, the growth of LTBMCC spheroids (containing 30-40 cells) was obtained. An essentially higher content of hematopoietic precursor cells (colony-forming units-granulocyte macrophage) was registered in the rotating-vessel system. In this bioreactor, a growth of large HD-MY-Z spheroids (containing 100-200 cells) was achieved. The composed

References

    Issue

    Conference on Signal Transduction Pathways, Chromatin Structures and Gene Expression Mechanisms as Therapeutic Targets (Science Week 2004) Location: Luxembourg, LUXEMBOURG Date: JAN 25-31, 2004 Book Series: ANNALS OF THE NEW YORK ACADEMY OF SCIENCES Volume: 1030 Pages: 103-115, ISSN 0077-8923, E-ISSN:1749-6632, pp. 103-115, 2004, United States,

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