Autors: Ivanova, N. V., Godjevargova Ts., Simeonova A., Konsulov V., Turmanova S.
Title: Behaviour of immobilized glucose oxidase on membranes from polyacrylonitrile and copolymer of methylmethacrylate-dichlorophenylmaleimide
Keywords: ultrafiltration membranes, polyacrylonitrile, glucose oxidas

Abstract: Two new ultrafiltration membranes were obtained from a polymer mixture, containing 60% polyacrylonitrile (PAN) and 40% copolymer of methylmethacrylate-dichlorophenylmaleimide (MMA-DCPMI). Membrane 1 (MB1) contains 40% DCPMI of the copolymer, and membrane 2 (MB2) contains 15% of the copolymer. The pore size, the specific surface, the water content, the water flux, and the selectivity were determined for the two membranes. The presence of dichlorophenylmaleimide in the copolymer ensures the preparation of membranes suitable for direct covalent enzyme immobilization without further modifications. These membranes were used for immobilization of glucose oxidase (GOD). High amount of bound protein was found on each of the membranes. High relative activities of the immobilized GOD were achieved, 72% for MB1 and 68% for MB2. The properties of the immobilized enzyme (GOD) were determined: optimum pH and temperature and pH, thermal, and storage stability, and then compared with the properties

References

    Issue

    Journal of Applied Polymer Science, vol. 101, issue 6, pp. 4334-4340, 2006, United States, © 2006 Wiley Periodicals, ISBN ISSN: 0021-8995

    Вид: статия в списание, публикация в издание с импакт фактор, индексирана в Scopus