Autors: Ivanova, N. V., Atanasova M., Godjevargova Tz.
Title: Determination of Aflatoxin M1 in Milk by a Magnetic Nanoparticle-Based Fluorescent Immunoassay
Keywords: Aflatoxin M1; fluorescence; immunoassay; magnetic nanopartic

Abstract: A sensitive and rapid magnetic nanoparticle-based fluorescent immunoassay for the determination of aflatoxin M1 in raw milk was developed. Aflatoxin M1 was converted to aflatoxin M1-ocarboxymethyl oxime. The aflatoxin M1-oxime was used for the preparation of aflatoxin M1-oxime-fluoresceinamine conjugate through the carbodiimide reaction. The aflatoxin M1-oxime-fluoresceinamine conjugate was characterized by ultraviolet–visible and infrared spectroscopy. Magnetic nanoparticles (Fe3O4) were synthesized and modified by 3-(aminopropyl)triethoxysilane. The size of initial (139 nm) and functionalized magnetic nanoparticles (147 nm) was determined by particle analysis. The optimal mass of immobilized antibody (25 μg) and optimal concentration of aflatoxin M1-oxime-fluoresceinamine conjugate (15 μg mL−1) for magnetic nanoparticle-based fluorescent immunoassay were determined. The developed immunoassay provided a linear aflatoxin M1 concentration range from 3.0 to 100 pg mL−1 in bovine milk. T

References

    Issue

    Analytical Letters, vol. 50, issue 3, pp. 452–469, 2017, United States, ISBN ISSN: 0003-2719 (Print) 1532-236X (Online)

    Вид: пленарен доклад в международен форум, публикация в издание с импакт фактор, публикация в реферирано издание, индексирана в Scopus