Autors: Ivanova, N. V., Ivanov Y., Damyanova S., Kostova I., Godjevargova Tz.
Title: Hydrolysis of whey lactose by immobilized β-galactosidase in a bioreactor with a spirally wound membrane
Keywords: -Galactosidase Whey lactose Reactor with a spirally wound me

Abstract: The -galactosidase was covalently immobilized onto a modified polypropylene membrane, using glutaraldehyde. The optimal conditions for hydrolysis of lactose (4.7%) by immobilized -galactosidase in a batch process were determined 13.6 U enzyme activity, 40 ◦C, pH 6.8 and 10 h. The obtained degree of hydrolysis was compared with results received by a free enzyme. It was found, that the lactose hydrolysis by an immobilized enzyme was 1.6 times more effective than the lactose hydrolysis by a free enzyme. It was determined that the stability of the immobilized enzyme was 2 times higher in comparison with the stability of free enzyme. The obtained immobilized system -galactosidase/polypropylene membrane was applied to produce glucose–galactose syrup from waste whey. The whey characteristics and the different preliminary treatments of the whey were investigated. Then the whey lactose hydrolysis in a bioreactor by an immobilized enzyme on a spirally wound membrane was performed. The optimal m

References

    Issue

    International journal of biological macromolecules, vol. 82, pp. 339 - 346, 2016, Netherlands, Elsevier, ISBN ISSN: 0141-8130

    Вид: статия в списание, публикация в издание с импакт фактор, публикация в реферирано издание, индексирана в Scopus