Autors: Ivanova, N. V., Iotov V., Ivanov Y., Godjevargova Tz., Kotia N.
Title: Immobilization of beta-galactosidase on modified polypropilene membranes
Keywords: -Galactosidase Immobilization Modification Polypropylene me

Abstract: A new immobilized system: -galactosidase-modified polypropylene membrane was created. It was obtained 13 different carriers by chemical modification of polypropylene membranes by two stages. The first stage is treatment with K2Cr2O7 to receive carboxylic groups on membrane surface. The second stage is treatment with different modified agents ethylendiamine, hexamethylenediamine, hydrazine dihydrochloride, hydroxylamine, o-phenylenediamine, p-phenylenediamine, N,N-dibenzyl ethylenediamine diacetate to receive amino groups. The quantity of the amino groups, carboxylic groups and the degree of hydrophilicity of unmodified and modified polypropilene membranes were determined. -Galactosidase was chemically immobilized on the obtained carries by glutaraldehyde. The highest relative activity of immobilized enzyme was recorded at membrane modified with 10% hexamethylenediamine (Membrane 5) – 92.77%. The properties of immobilized -galactosidase on different modified membranes – pH optimum,

References

    Issue

    International Journal of Biological Macromolecules, vol. 51, pp. 710-719, 2012, Netherlands, Elsevier, http://dx.doi.org/10.1016/j.ijbiomac.2012.07.032

    Вид: статия в списание, публикация в издание с импакт фактор, публикация в реферирано издание, индексирана в Scopus