| Autors: Marinov I., Ivanov Y., Ivanova, N. V., Godjevargova Ts. Title: Amperometric inhibition-based detection of organophosphorus pesticides in unary and binary mixtures employing flow-injection analysis Keywords: Amperometric biosensor Immobilized acetylcholinesterase Pest Abstract: The present work is focused on the application of an acetylthiocholine (ATCh) biosensor in a flowinjection system for the detection of organophosphorus pesticides. The optimal operating conditions of the flow-injection system were determined: flow-rate – 0.5 mL min−1, substrate concentration – 100 M, incubation and reactivation time–10 min. A calibration plot was obtained for ATCh concentration ranging from 20 to 200 M. A linear interval was detected along the calibration curve from 20 to 100 М with a correlation coefficient R2 = 0.996. The sensitivity of the constructed biosensor was calculated to be 0.083 A M−1 cm−2. The application of the flow-injection system for detection and quantification of three organophosphorus pesticides – paraoxon ethyl, monocrotophos and dichlorvos in unary solutions and in binary mixtures was investigated as well. The inhibition curves for each pesticide was plotted and the linear intervals were determined along with the corresponding equations and References Issue
|
Вид: статия в списание, публикация в издание с импакт фактор, публикация в реферирано издание, индексирана в Scopus
