Autors: Ivanova, N. V., Atanasova M. K., Godjevargova T. I.
Title: Enzyme-linked immunosorbent assay for determination of aflatoxin M1 based on magnetic nanoparticles
Keywords: Aflatoxin M1; Antibody; Magnetic nanoparticles; Immunoassay

Abstract: A sensitive enzyme immunoassay with magnetic nanoparticles (Method A) for the quantitative determination of aflatoxin M1 in milk was developed. This immunoassay was based on the immobilization of monoclonal antibody (mAb) on the modified magnetic nanoparticles (MNPs-NH2). It was observed that for each mg of the MNPs, 25 ????g of antibody was immobilized. Both aflatoxin M1 in the sample and aflatoxin M1-BSA-peroxidase conjugate competed for the immobilized antibody. The proposed Method A was compared with other method (B). The Method B was based on the immobilization of aflatoxin M1-BSA conjugate on the MNPs-NH2, which competed with the aflatoxin M1 in the sample for binding to the added mAb. The binding of mAb to the aflatoxin M1-BSA-MNPs-NH2 was detected using a target secondary IgG-peroxidase antibody. The analytical characteristics of the two methods were compared. Real milk samples were investigated for present of aflatoxin M1. Two methods were based on the use of MNPs as a solid su

References

    Issue

    The 6th International Advances in Applied Physics and Materials Science Congress & Exhibition, (APMAS 2016), 1–3 June 2016, AIP Conference Proceedings, (2017), vol. 1809, issue 1, pp. 020005-1 – 020005-12, 2017, Turkey, Published by AIP Publishing, https://doi.org/10.1063/1.4975420

    Вид: пленарен доклад в международен форум, публикация в реферирано издание, индексирана в Scopus и Web of Science